BioPlex - Human Interactome

The human interactome is composed on thousands of interactions and protein communities. Using a robust interaction proteomics platform, we - together with the Gygi lab - have performed interaction proteomics on more than 17,000 human proteins in HEK293T cells and 10,000 proteins in HCT116 cells. This has led to now three iterations of the BioPlex database of interacting proteins (available at https://bioplex.hms.harvard.edu/) [Cell, 2015, Nature, 2017, Cell, 2021]. Current efforts seek to convert these interaction networks into structural models for the proteome using Alphafold, with more than 100,000 predictions performed thus far. The creation of a structural interactome will fuel numerous hypothesis-driven experiments to elucidate functions of poorly understood proteins, often referred to as the “dark proteome”.